the effect of allium sativum (garlic) extract on infectious bronchitis virus in specific pathogen free embryonic egg

objective: garlic is a plant has been used as a flavor, and anti-microbial and anti-diarrheal agent. infectious bronchitis virus (ibv) is a coronavirus. the available vaccines against ibv cannot cover new variants. this study evaluated the inhibitory effects of garlic extract on ibv.  materials and methods: the constituents of garlic extract were detected by gas chromatography. this study was done in four groups of embryonic spf eggs; first group was used for virus titration; second group received the mixture of different virus titration and constant amount of garlic extract; third group received 10-3 titration of virus and after 8 hr received garlic extract and the last group received different dilutions of garlic extract. results: based on our results, in the second group, ibv vaccine strain (4/91) at all titration and m41 in 10-2 and 10-3 titration and in the third group both variants of virus the embryonic index (ei) was significantly increased. conclusion: the garlic extract had inhibitory effects on ibv in the chickens embryo.